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phosphor mtor  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc phosphor mtor
    Phosphor Mtor, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phosphor+mtor/us12545666-1153-33-35
    Average 86 stars, based on 1 article reviews
    phosphor mtor - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Incubation:

    Article Title: GenX induces neuroinflammatory responses in BV2 microglial cells through mTOR signaling-mediated inhibition of autophagy.
    Article Snippet: Proteins were resolved via SDS-PAGE using 8 %, 10 %, or 15 % polyacrylamide gels and subsequently blotted onto PVDF membranes (Thermo Fisher Scientific, Waltham, MA, USA). .. Membranes underwent overnight incubation at 4◦C with primary antibodies targeting LC3 (#4108, Cell Signaling), BECN1 (ab62557, Abcam), SQSTM1 (ab56416, Abcam), ATG5 (#12994, Cell Signaling), PI3K (SC-376112, Santa Cruz), AKT (#9272, Cell Signaling), phosphor-AKT (#9271, Cell Signaling), mTOR (#2972, Cell Signaling), phosphor-mTOR (#5536, Cell Signaling), phosphor-4EBP1 (SC-293124, Santa Cruz), phosphor- Ribosomal Protein S6 (SC-293144, Santa Cruz), IBA1 (ab178846, abcam), CD11B (ab133357, abcam) and β-actin (SC-517582, Santa Cruz). .. Visualization utilized HRP-linked secondary antibodies (Thermo Fisher Scientific, Waltham, MA, USA) and ECL substrate (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Panduratin A Induces Autophagy Through AMPK Activation Independent of mTOR Inhibition and Restricts Mycobacterium tuberculosis in Host Macrophages
    Article Snippet: Equal amounts of protein were separated by 15% SDS‐polyacrylamide gel electrophoresis at 70 V for 2 h, followed by transfer onto PVDF membranes (Bio‐Rad). .. Membranes were blocked with 5% skimmed milk for 1 h and incubated overnight at 4°C with primary antibodies against p62 (Progen, 1:3000), LC3 (MBL International, 1:2000), phosphor‐mTOR (Cell Signaling, 1:1000), mTOR (Cell Signaling, 1:1000), phosphor‐4E‐BP1 (Cell Signaling, 1:1000), 4E‐BP1 (Cell Signaling, 1:1000), phosphor‐AMPKα (Cell Signaling, 1:500), AMPKα (Cell Signaling, 1:1000), and Actin (Abcam, 1:10,000). ..

    Article Title: Repression of Polycomb Group Factor 1 Inhibits EMT Progression in Colon Cancers Cells via Wnt/β-Catenin and PI3K/Akt/mTOR Signaling Pathway.
    Article Snippet: .. Skim milk (5%) was used to seal the membrane at room temperature for 1 h, and the primary antibody was diluted at 1:1000 and incubated overnight at 4°C (PCGF1, ab259943, Abcam, RRID: AB_10672187; β-actin, ab8827, Abcam, RRID: AB_2305186; N-cadherin, 22,018–1-AP, proteintech, RRID: AB_2813891; E-cadherin, 20,874–1-AP, proteintech, RRID: AB_10697811; Snail-1, 13,099–1-AP, proteintech, RRID: AB_2191756; mTOR, 2972, Cell Signaling Technology, RRID: AB_330978; phosphor-mTOR [Ser2448], 2971, Cell Signaling Technology, RRID: AB_330970; Akt, 9272, Cell Signaling Technology, RRID: AB_329827; phosphor-Akt [Ser473], 9271, Cell Signaling Technology, RRID: AB_329825; PI3K, Cell Signaling Technology, 4292, RRID: AB_329869; Phospho-PI3K, Cell Signaling Technology, 17,366, RRID: AB_2895293; β-catenin, 51,067–2-AP, Proteintech, RRID: AB_2086128; c-Myc, 10,828–1-AP, Proteintech, RRID: AB_2148585).After washing the membrane three times, it was incubated with HRP-labeled secondary antibodies (1:5000, RGAR001, proteintech, RRID: AB_3073505) for 1 h at room temperature. .. After washing the film three times, it was developed using ECL Plus (Amersham Pharmacia Biotech, USA) for chemiluminescence color development.

    Article Title: Panduratin A Induces Autophagy Through AMPK Activation Independent of mTOR Inhibition and Restricts Mycobacterium tuberculosis in Host Macrophages.
    Article Snippet: Tuberculosis (TB), caused by Mycobacterium tuberculosis (Mtb), remains a major global health burden, especially with the increasing prevalence of drugresistant strains.. There is an urgent need for new therapeutics that act via alternative mechanisms.. Autophagy, a vital cellautonomous defense process, allows macrophages to degrade intracellular pathogens such as Mtb and has gained attention as a potential target for hostdirected therapy.

    other:

    Article Title: Effect of the G-Protein-Coupled Receptor T2R14 on Proliferation and Cell Population Growth in Oral Cancer Cells
    Article Snippet: Primary antibodies against proliferating cell nuclear antigen (PCNA) (#13110), mTOR (#2983), and phosphor-mTOR (#5536) were purchased from Cell Signaling Technology (Whitby, ON, Canada).

    Article Title: Effect of the G-Protein-Coupled Receptor T2R14 on Proliferation and Cell Population Growth in Oral Cancer Cells
    Article Snippet: Primary antibodies against proliferating cell nuclear antigen (PCNA) (#13110), mTOR (#2983), and phosphor-mTOR (#5536) were purchased from Cell Signaling Technology (Whitby, ON, Canada).

    Membrane:

    Article Title: Repression of Polycomb Group Factor 1 Inhibits EMT Progression in Colon Cancers Cells via Wnt/β-Catenin and PI3K/Akt/mTOR Signaling Pathway.
    Article Snippet: .. Skim milk (5%) was used to seal the membrane at room temperature for 1 h, and the primary antibody was diluted at 1:1000 and incubated overnight at 4°C (PCGF1, ab259943, Abcam, RRID: AB_10672187; β-actin, ab8827, Abcam, RRID: AB_2305186; N-cadherin, 22,018–1-AP, proteintech, RRID: AB_2813891; E-cadherin, 20,874–1-AP, proteintech, RRID: AB_10697811; Snail-1, 13,099–1-AP, proteintech, RRID: AB_2191756; mTOR, 2972, Cell Signaling Technology, RRID: AB_330978; phosphor-mTOR [Ser2448], 2971, Cell Signaling Technology, RRID: AB_330970; Akt, 9272, Cell Signaling Technology, RRID: AB_329827; phosphor-Akt [Ser473], 9271, Cell Signaling Technology, RRID: AB_329825; PI3K, Cell Signaling Technology, 4292, RRID: AB_329869; Phospho-PI3K, Cell Signaling Technology, 17,366, RRID: AB_2895293; β-catenin, 51,067–2-AP, Proteintech, RRID: AB_2086128; c-Myc, 10,828–1-AP, Proteintech, RRID: AB_2148585).After washing the membrane three times, it was incubated with HRP-labeled secondary antibodies (1:5000, RGAR001, proteintech, RRID: AB_3073505) for 1 h at room temperature. .. After washing the film three times, it was developed using ECL Plus (Amersham Pharmacia Biotech, USA) for chemiluminescence color development.



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